Source code for dnacauldron.Assembly.builtin_assembly_classes.LigaseCyclingReactionAssembly

from ...AssemblyMix.LigaseCyclingReactionMix import LigaseCyclingReactionMix
from ...AssemblyMix import AssemblyMixError
from ...Fragment.HomologousFragment import HomologyChecker
from ..AssemblySimulation import AssemblySimulation
from ..AssemblyFlaw import AssemblyFlaw
from ..Assembly import Assembly

[docs]class LigaseCyclingReactionAssembly(Assembly): """Representation and simulation of Gibson Assembly Parameters ---------- parts A list of parts names corresponding to records in a repository. bridging oligo names can also be provided in this part list, however in that case ``bridging_olios`` should be an empty list and an ``oligo_indicator`` string should be provided. homology_checker An HomologyChecker instance defining which homology sizes and melting temperatures are valid between one bridging oligo and one part. bridging_oligos A list of the name of bridging oligos if they are not included in the part names oligos_indicator String to use to identify bridging oligos when these are provided mixed with the other parts. The string should be common to all oligo names but should not appear in any part name. For instance "BO_". name Name of the assembly as it will appear in reports. max_constructs None or a number of maximum assemblies to compute (avoids complete freeze for combinatorial assemblies with extremely many possibilities). expected_constructs Either a number or a string ``'any_number'``. If the number of constructs doesn't match this value, the assembly will be considered invalid in reports and summaries expect_no_unused_parts If True and some parts are unused, this will be considered an invalid assembly in summaries and reports. dependencies (do not use). Metadata indicating which assemblies depend on this assembly, or are depended on by it. """ spreadsheet_import_parameters = ( "level", "expected_constructs", "expect_no_unused_parts", "connectors_collection", "oligo_indicator" ) def __init__( self, parts, bridging_oligos=(), oligo_indicator=None, homology_checker="default", name="homologous_assembly", connectors_collection=None, expected_constructs=1, expect_no_unused_parts=True, max_constructs=40, dependencies=None, ): self.oligo_indicator = oligo_indicator if oligo_indicator is not None: bridging_oligos = [ name for name in parts if oligo_indicator in name ] parts = [name for name in parts if oligo_indicator not in name] self.parts = parts self.bridging_oligos = bridging_oligos Assembly.__init__( self, name=name, parts=parts, max_constructs=max_constructs, dependencies=dependencies, ) self.connectors_collection = connectors_collection self.expected_constructs = expected_constructs self.expect_no_unused_parts = expect_no_unused_parts if homology_checker == "default": homology_checker = HomologyChecker() self.homology_checker = homology_checker def get_extra_construct_data(self): description = self.homology_checker.parameters_as_string() return dict(homology_condition=description) def _detect_unused_parts_error(self, construct_records): used_parts = [ fragment.original_part.id for construct_record in construct_records for fragment in construct_record.fragments ] unused_parts = set(self.parts).difference(set(used_parts)) if len(unused_parts): if len(construct_records): suggestion = "Check assembly plan" else: suggestion = "Check parts design" return AssemblyFlaw( assembly=self, message="Some parts are unused", suggestion=suggestion, data={"unused_parts": sorted(unused_parts)}, ) def _detect_parts_connections_errors(self, assembly_mix): warnings = [] graph = assembly_mix.connections_graph fragment_degrees = { fragment: graph.degree(fragment) for fragment in graph.nodes() } # CHECK PARTS WITH A SINGLE OVERHANG deadend_parts = [ assembly_mix.fragments_dict[fragment].original_part.id for fragment, degree in fragment_degrees.items() if degree == 1 ] deadend_parts = sorted(set(deadend_parts)) if len(deadend_parts): warning = AssemblyFlaw( assembly=self, message="Part(s) with single-sided sticky end", suggestion="Check restriction sites or assembly plan", data={"parts": deadend_parts}, ) warnings.append(warning) # CHECK PARTS WITH MORE THAN 2 SLOT CONNECTIONS (FORK/CROSSROAD) fork_parts = [ assembly_mix.fragments_dict[fragment].original_part.id for fragment, degree in fragment_degrees.items() if degree > 2 ] fork_parts = sorted(set(fork_parts)) if len(fork_parts): warning = AssemblyFlaw( assembly=self, message="Warning: parts at graph forking positions", suggestion="Check restriction sites in part sequences", data={"parts": fork_parts}, ) warnings.append(warning) return warnings
[docs] def simulate(self, sequence_repository, annotate_parts_homologies=True): """Simulate the Gibson Assembly, return an AssemblySimulation.""" # CREATE THE MIX warnings = [] parts_records = sequence_repository.get_records(self.parts) oligos_records = sequence_repository.get_records(self.bridging_oligos) mix = LigaseCyclingReactionMix( parts=parts_records, bridging_oligos=oligos_records, homology_checker=self.homology_checker, name=self.name + "_homology_mix", ) # ATTEMPT CONNECTOR AUTOSELECTION IF NECESSARY connectors_records = self._get_connectors_records(sequence_repository) if len(connectors_records) != 0: try: connectors = mix.autoselect_connectors(connectors_records) if len(connectors): connectors_ids = [c.id for c in connectors] connectors_string = " & ".join(connectors_ids) warning = AssemblyFlaw( assembly=self, message="Added connectors %s" % connectors_string, suggestion="", data={"selected_connectors": connectors_ids}, ) warnings.append(warning) except AssemblyMixError as err: error = AssemblyFlaw( assembly=self, message="Failed to find suitable connectors", suggestion="Check assembly plan or parts design", ) return AssemblySimulation( assembly=self, construct_records=[], errors=[error], sequence_repository=sequence_repository, ) # COMPUTE ALL CIRCULAR ASSEMBLIES generator = mix.compute_circular_assemblies( annotate_parts_homologies=annotate_parts_homologies ) construct_records = sorted( [asm for (i, asm) in zip(range(self.max_constructs), generator)], key=lambda asm: str(asm.seq), ) self.attribute_ids_to_constructs(construct_records) # FIND ALL ERRORS errors = [] found = len(construct_records) self._detect_constructs_number_error(found, errors) self._detect_max_constructs_reached(found, warnings) if self.expect_no_unused_parts: unused_error = self._detect_unused_parts_error(construct_records) if unused_error is not None: errors.append(unused_error) if errors != []: warnings.extend(self._detect_parts_connections_errors(mix)) return AssemblySimulation( assembly=self, construct_records=construct_records, mixes=(mix,), errors=errors, warnings=warnings, sequence_repository=sequence_repository, )